| Sep 26, 2021 | Southern 38, Mississippi Valley State 25 | Box score |
| Sep 30, 2021 | Ashland 35, Walsh 10 | Box score |
| Sep 30, 2021 | Prairie View A&M 27, Arkansas–Pine Bluff 17 | Box score |
| Oct 1, 2021 | Dartmouth 31, Pennsylvania 7 | Box score |
| Oct 1, 2021 | Southern Connecticut State 23, American International 7 | Box score |
| Oct 2, 2021 | Rhode Island 27, Stony Brook 20 | Box score |
| Oct 2, 2021 | Saint Francis 27, Morgan State 14 | Box score |
| Oct 2, 2021 | Drake 6, Butler 3 | Box score |
| Oct 2, 2021 | New Haven 28, Pace 14 | Box score |
| Oct 2, 2021 | Shepherd 59, Shippensburg 27 | Box score |
| Oct 2, 2021 | Millersville 31, Lock Haven 29 | Box score |
| Oct 2, 2021 | Charleston (WV) 38, Notre Dame College 28 | Box score |
| Oct 2, 2021 | Yale 34, Lehigh 0 | Box score |
| Oct 2, 2021 | Kutztown 21, East Stroudsburg 14 | Box score |
| Oct 2, 2021 | Colgate 28, Georgetown 21 | Box score |
| Oct 2, 2021 | Shaw 27, Virginia Union 7 | Box score |
| Oct 2, 2021 | Monmouth 54, Gardner-Webb 17 | Box score |
| Oct 2, 2021 | North Carolina A&T 41, Robert Morris 14 | Box score |
| Oct 2, 2021 | Princeton 24, Columbia 7 | Box score |
| Oct 2, 2021 | Ouachita Baptist 64, Northwestern (OK) 17 | Box score |
| Oct 2, 2021 | Marist 27, Valparaiso 24 | Box score |
| Oct 2, 2021 | SE Louisiana 38, McNeese 35 | Box score |
| Oct 2, 2021 | Michigan Tech 31, Davenport 7 | Box score |
| Oct 2, 2021 | Ferris State 67, Northwood 25 | Box score |
| Oct 2, 2021 | Savannah State 21, Kentucky State 17 | Box score |
| Oct 2, 2021 | Minnesota Duluth 26, Minnesota St Moorhead 21 | Box score |
| Oct 2, 2021 | Allen 46, Central State 34 | Box score |
| Oct 2, 2021 | Duquesne 37, Merrimack 14 | Box score |
| Oct 2, 2021 | West Liberty 20, Glenville State 19 | Box score |
| Oct 2, 2021 | Mars Hill 28, Newberry 22 | Box score |
| Oct 2, 2021 | Howard 22, Sacred Heart 17upset | Box score |
| Oct 2, 2021 | Ohio Dominican 32, Kentucky Wesleyan 30 | Box score |
| Oct 2, 2021 | Frostburg State 51, UNC Pembroke 21 | Box score |
| Oct 2, 2021 | Assumption 35, Franklin Pierce 28 | Box score |
| Oct 2, 2021 | Davidson 35, Stetson 28 | Box score |
| Oct 2, 2021 | South Carolina State 42, Bethune-Cookman 35 | Box score |
| Oct 2, 2021 | Harvard 38, Holy Cross 13 | Box score |
| Oct 2, 2021 | Chattanooga 45, Western Carolina 17 | Box score |
| Oct 2, 2021 | Bowie State 44, Johnson C. Smith 7 | Box score |
| Oct 2, 2021 | Norfolk State 47, Hampton 44 | Box score |
| Oct 2, 2021 | Elon 20, Richmond 7upset | Box score |
| Oct 2, 2021 | Arkansas Tech 51, Southern Nazarene 41 | Box score |
| Oct 2, 2021 | Missouri State 41, Illinois State 20 | Box score |
| Oct 2, 2021 | Morehead State 45, Dayton 38 | Box score |
| Oct 2, 2021 | The Citadel 35, VMI 24 | Box score |
| Oct 2, 2021 | Quincy 31, Missouri S&T 28 | Box score |
| Oct 2, 2021 | Indianapolis 77, Southwest Baptist 10 | Box score |
| Oct 2, 2021 | Grand Valley State 49, Saginaw Valley State 17 | Box score |
| Oct 2, 2021 | Wayne State (NE) 38, Winona State 21 | Box score |
| Oct 2, 2021 | Fort Valley State 24, Benedict 14 | Box score |
| Oct 2, 2021 | Tuskegee 21, Clark Atlanta 0 | Box score |
| Oct 2, 2021 | Edward Waters 37, Morehouse 13 | Box score |
| Oct 2, 2021 | Sioux Falls 28, Augustana (SD) 26 | Box score |
| Oct 2, 2021 | West Chester 21, Bloomsburg 20 | Box score |
| Oct 2, 2021 | IU Pennsylvania 28, Gannon 23 | Box score |
| Oct 2, 2021 | Black Hills State 45, Fort Lewis 17 | Box score |
| Oct 2, 2021 | Chadron State 46, New Mexico Highlands 16 | Box score |
| Oct 2, 2021 | Colorado School of Mines 20, Western Colorado 14 | Box score |
| Oct 2, 2021 | Emporia State 35, Washburn 30 | Box score |
| Oct 2, 2021 | UVA Wise 35, Lenoir Rhyne 28 | Box score |
| Oct 2, 2021 | Barton 31, Carson Newman 17 | Box score |
| Oct 2, 2021 | Fairmont State 31, Concord 27 | Box score |
| Oct 2, 2021 | Wheeling 38, West Virginia Wesleyan 0 | Box score |
| Oct 2, 2021 | Erskine 59, Virginia Lynchburg 13 | Box score |
| Oct 2, 2021 | Elizabeth City State 19, Livingstone 13 | Box score |
| Oct 2, 2021 | Tennessee Tech 28, Southeast Missouri State 17 | Box score |
| Oct 2, 2021 | Eastern Kentucky 20, Tarleton State 3 | Box score |
| Oct 2, 2021 | Kennesaw State 31, Jacksonville State 6 | Box score |
| Oct 2, 2021 | Montana State 40, Northern Colorado 7 | Box score |
| Oct 2, 2021 | Delaware 20, UAlbany 15 | Box score |
| Oct 2, 2021 | Arkansas Monticello 18, Southwestern Oklahoma State 16 | Box score |
| Oct 2, 2021 | South Dakota 38, Indiana State 10 | Box score |
| Oct 2, 2021 | East Central 38, Southern Arkansas 17 | Box score |
| Oct 2, 2021 | North Dakota State 16, North Dakota 10 | Box score |
| Oct 2, 2021 | Harding 31, Oklahoma Baptist 7 | Box score |
| Oct 2, 2021 | Henderson State 27, Southeastern Oklahoma State 24 | Box score |
| Oct 2, 2021 | Grambling 37, Alabama A&M 28upset | Box score |
| Oct 2, 2021 | Mississippi College 42, North Greenville 21upset | Box score |
| Oct 2, 2021 | South Dakota Mines 45, Adams State 42 | Box score |
| Oct 2, 2021 | University of Mary 49, Northern State 42upset | Box score |
| Oct 2, 2021 | Lane College 71, Texas College 0 | Box score |
| Oct 2, 2021 | Minnesota State 58, Concordia St. Paul 10 | Box score |
| Oct 2, 2021 | Central Missouri 41, Northeastern State 10 | Box score |
| Oct 2, 2021 | Central Oklahoma 69, Lincoln (MO) 28 | Box score |
| Oct 2, 2021 | Pittsburg State 20, Missouri Southern State 16 | Box score |
| Oct 2, 2021 | Findlay 37, Lake Erie 10 | Box score |
| Oct 2, 2021 | Bemidji State 49, Minot State 35 | Box score |
| Oct 2, 2021 | California (PA) 37, Mercyhurst 0 | Box score |
| Oct 2, 2021 | James Madison 23, New Hampshire 21 | Box score |
| Oct 2, 2021 | Bucknell 21, Cornell 10 | Box score |
| Oct 2, 2021 | East Tennessee State 27, Wofford 21 | Box score |
| Oct 2, 2021 | Bentley 34, Saint Anselm 21 | Box score |
| Oct 2, 2021 | Fordham 42, Lafayette 41 | Box score |
| Oct 2, 2021 | Northern Arizona 48, Idaho State 17 | Box score |
| Oct 2, 2021 | Mississippi Valley State 17, North Carolina Central 16 | Box score |
| Oct 2, 2021 | Southern Illinois 31, Western Illinois 30 | Box score |
| Oct 2, 2021 | Nicholls 48, Houston Christian 17 | Box score |
| Oct 2, 2021 | Northern Michigan 26, Wayne State (MI) 19 | Box score |
| Oct 2, 2021 | Edinboro 17, Seton Hill 7 | Box score |
| Oct 2, 2021 | CSU Pueblo 19, Colorado Mesa 13 | Box score |
| Oct 2, 2021 | Hillsdale 24, Tiffin 21upset | Box score |
| Oct 2, 2021 | Southwest Minnesota State 20, Upper Iowa 14 | Box score |
| Oct 2, 2021 | San Diego 27, St. Thomas (MN) 24 | Box score |
| Oct 2, 2021 | Bryant 36, Brown 29 | Box score |
| Oct 2, 2021 | Western Oregon 41, West Texas 38 | Box score |
| Oct 2, 2021 | Sam Houston 21, Stephen F. Austin 20 | Box score |
| Oct 2, 2021 | Delaware State 33, Wagner 27 | Box score |
| Oct 2, 2021 | Northern Iowa 34, Youngstown State 7 | Box score |
| Oct 2, 2021 | Murray State 22, Eastern Illinois 6 | Box score |
| Oct 2, 2021 | Virginia State 33, St. Augustine’s 9 | Box score |
| Oct 2, 2021 | Chowan 73, Winston-Salem State 7 | Box score |
| Oct 2, 2021 | Mercer 45, Samford 42 | Box score |
| Oct 2, 2021 | Florida A&M 28, Alabama State 0 | Box score |
| Oct 2, 2021 | West Alabama 38, West Georgia 20 | Box score |
| Oct 2, 2021 | Slippery Rock 75, Clarion 0 | Box score |
| Oct 2, 2021 | Wingate 43, Tusculum 35 | Box score |
| Oct 2, 2021 | Fayetteville State 46, Lincoln (PA) 9 | Box score |
| Oct 2, 2021 | Central Arkansas 42, Abilene Christian 21 | Box score |
| Oct 2, 2021 | South Dakota State 55, Utah Tech 7 | Box score |
| Oct 2, 2021 | Campbell 48, North Alabama 31 | Box score |
| Oct 2, 2021 | Incarnate Word 38, Northwestern State 27 | Box score |
| Oct 2, 2021 | Lindenwood 31, Truman State 21upset | Box score |
| Oct 2, 2021 | Albany State 31, Miles 3 | Box score |
| Oct 2, 2021 | Valdosta State 49, Shorter 13 | Box score |
| Oct 2, 2021 | West Florida 39, Delta State 33 | Box score |
| Oct 2, 2021 | Angelo State 62, Western New Mexico 3 | Box score |
| Oct 2, 2021 | East Texas A&M 72, UFTL 6 | Box score |
| Oct 2, 2021 | Northwest Missouri State 30, Missouri Western 7 | Box score |
| Oct 2, 2021 | West Virginia State 45, Alderson-Broaddus 13 | Box score |
| Oct 2, 2021 | Portland State 20, Southern Utah 13 | Box score |
| Oct 2, 2021 | Tennessee State 24, Austin Peay 22upset | Box score |
| Oct 2, 2021 | Fort Hays 42, Nebraska Kearney 35 | Box score |
| Oct 2, 2021 | Eastern New Mexico 52, Lincoln (CA) 6 | Box score |
| Oct 2, 2021 | Weber State 38, Cal Poly 7 | Box score |
| Oct 2, 2021 | Central Washington 30, Midwestern State 20 | Box score |
| Oct 2, 2021 | UC Davis 27, Idaho 20 | Box score |
| Oct 2, 2021 | Eastern Washington 34, Montana 28 | Box score |